Journal: Cell Communication and Signaling : CCS

Article Title: Jumu is required for circulating hemocyte differentiation and phagocytosis in Drosophila

doi: 10.1186/s12964-018-0305-3

Figure Lengend Snippet: Expression levels of proteins associated with actin filopodium formation are changed in jumu knockdown hemocytes. a Immunostaining against Ena (red) and Fascin (red) shows that the expression of Ena and Fascin is reduced in jumu knockdown hemocytes compared with the expression in the controls; however, the knockdown of NimC1 does not affect the expression of Ena and Fascin. b, c Quantification of signal intensities. d Real-time PCR analysis of ena , fascin , profilin and rho1 levels in jumu knockdown hemocytes. e-g Phalloidin staining (green) shows that the number and length of filopodia are reduced in the circulating hemocytes of Hml > GFP > ena RNAi and Hml > GFP > sn RNAi . h-j Circulating hemocytes of Hml > GFP > ena RNAi and Hml > GFP > sn RNAi isolated from third-instar larvae injected with latex beads (red) 1 h postinjection show defects in filopodia (green). k, l Quantification of the percentage of engulfing cells and phagocytic indexes based on phagocytosis assays. m-o’ Immunostaining against Ena (red) and phalloidin staining (green) shows that Ena is enriched at the tips of filopodia and lamellipodia in control circulating hemocytes; however, the expression level of Ena is markedly reduced at the tips of filopodia and lamellipodia in Hml > GFP > jumu RNAi (n and n’) and Hml > GFP > NimC1 RNAi (o and o’) circulating hemocytes. Error bars represent the S.E.M of at least 3 independent experiments; NS, not significant; *P < 0.05; **P < 0.01; ***P < 0.001 (Student’s t-test). Scale bars: 20 μm (a); 10 μm (e-j, m-o’)

Article Snippet: The following primary antibodies were used: mouse anti-NimC1, mouse anti-L1 and mouse anti-H2 (gifts from I. Ando); rat anti-Jumu (made in our lab); mouse anti-α-tubulin (sigma); rabbit anti-PH3 (Millipore); mouse anti-Dorsal, mouse anti-Ena, mouse anti-Fascin, mouse anti-Rho1 and mouse anti-Profilin (Developmental Studies Hybridoma Bank); rabbit anti-Dif (gift from Dominique Ferrandon).

Techniques: Expressing, Immunostaining, Real-time Polymerase Chain Reaction, Staining, Isolation, Injection

Journal: Cell Communication and Signaling : CCS

Article Title: Jumu is required for circulating hemocyte differentiation and phagocytosis in Drosophila

doi: 10.1186/s12964-018-0305-3

Figure Lengend Snippet: Overexpression of jumu induces cell spreading and large numbers of filopodia in hemocytes. a-d Phalloidin staining (green) shows that Hml > GFP > UAS-jumu displays an increased number of filopodia and larger lamellipodia than the control ( a and b ); knockdown of ena or fascin in Hml > GFP > UAS-jumu can inhibit cell spreading and the formation of numerous filopodia ( c and d ). e-g Quantification of filopodium numbers and lengths and lamellipodial area. h Quantification of the phagocytosis indexes for latex beads, B. bassiana , S. aureus or E. coli. i-l Quantification of Ena, Fascin, Rho1 and Profilin levels. m Western blot analysis using antibodies against Ena, Fascin, Profilin and Rho1 and the corresponding graphical representation show that the levels of Ena and Fascin are increased in jumu -overexpressing S2 cells compared with the expression in the controls, whereas the levels of Profilin are reduced and the levels of Rho1 are unchanged. Error bars represent the S.E.M of at least 3 independent experiments; NS, not significant; *P < 0.05; **P < 0.01; ***P < 0.001 (Student’s t- test). Scale bars: 10 μm

Article Snippet: The following primary antibodies were used: mouse anti-NimC1, mouse anti-L1 and mouse anti-H2 (gifts from I. Ando); rat anti-Jumu (made in our lab); mouse anti-α-tubulin (sigma); rabbit anti-PH3 (Millipore); mouse anti-Dorsal, mouse anti-Ena, mouse anti-Fascin, mouse anti-Rho1 and mouse anti-Profilin (Developmental Studies Hybridoma Bank); rabbit anti-Dif (gift from Dominique Ferrandon).

Techniques: Over Expression, Staining, Western Blot, Expressing

Journal: Cell Communication and Signaling : CCS

Article Title: Jumu is required for circulating hemocyte differentiation and phagocytosis in Drosophila

doi: 10.1186/s12964-018-0305-3

Figure Lengend Snippet: Model of the functions of Jumu in filopodium formation and circulating hemocyte phagocytosis. a Normal expression of Jumu maintains the levels of NimC1, Enabled and Fascin in a direct or indirect manner. Enabled and Fascin participate in filopodium formation. The phagocytosis receptor NimC1 is involved in the recognition of pathogens and the subcellular localization of Ena. b Deficiency of Jumu reduces the protein levels of NimC1, Enabled and Fascin and consequently inhibits filopodium formation and hemocyte phagocytosis. c Severe deficiency of jumu can inhibit normal hemocyte mitosis and result in enlarged multinucleated hemocytes. The loss of jumu may control the cell cycle and mitosis process by affecting the expression of the Cyclin genes png and piwi . Severe deficiency of jumu also induces the generation of lamellocytes through the activation of the Toll signaling pathway

Article Snippet: The following primary antibodies were used: mouse anti-NimC1, mouse anti-L1 and mouse anti-H2 (gifts from I. Ando); rat anti-Jumu (made in our lab); mouse anti-α-tubulin (sigma); rabbit anti-PH3 (Millipore); mouse anti-Dorsal, mouse anti-Ena, mouse anti-Fascin, mouse anti-Rho1 and mouse anti-Profilin (Developmental Studies Hybridoma Bank); rabbit anti-Dif (gift from Dominique Ferrandon).

Techniques: Expressing, Activation Assay

Journal: Saudi Journal of Biological Sciences

Article Title: Histological changes in placental rat apoptosis via FasL and cytochrome c by the nano-herbal Zanthoxylum acanthopodium

doi: 10.1016/j.sjbs.2021.02.047

Figure Lengend Snippet: Histology of rats placental without treatments (C-l: A. Cytochrome c (50 µm), B. FasL (50 µm), C. Apoptosis (50 µm). D. Cytochrome c (16 µm), E. FasL (16 µm), F. Apoptosis (16 µm). EC: Epithelial Cells, FV: Fetal vessels.

Article Snippet: FasL detection used Fas-L mouse monoclonal antibodies (NOK-1):sc-19681 (dilution 1:50 with PBS, Santa Crus Biotechnology, Santa Cruz, CA, USA), and cytochrome c detection used a monoclonal mouse anti-cytochrome C antibody (ready to use) 7H8.2C12 (Medaysis Enable Innovation Company), formulation in PBS pH 7.4, containing BSA and ≤0.09% sodium aide (NaN 3 ).

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: Histological changes in placental rat apoptosis via FasL and cytochrome c by the nano-herbal Zanthoxylum acanthopodium

doi: 10.1016/j.sjbs.2021.02.047

Figure Lengend Snippet: Histology of hypertension rats placental (C+): A. Cytochrome c (20 µm), B. FasL (20 µm), C. Apoptosis (20 µm). D. Cytochrome c (20 µm), E. FasL (20 µm), F. Apoptosis (20 µm). EC: Epithelial Cells, FV: Fetal vessels.

Article Snippet: FasL detection used Fas-L mouse monoclonal antibodies (NOK-1):sc-19681 (dilution 1:50 with PBS, Santa Crus Biotechnology, Santa Cruz, CA, USA), and cytochrome c detection used a monoclonal mouse anti-cytochrome C antibody (ready to use) 7H8.2C12 (Medaysis Enable Innovation Company), formulation in PBS pH 7.4, containing BSA and ≤0.09% sodium aide (NaN 3 ).

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: Histological changes in placental rat apoptosis via FasL and cytochrome c by the nano-herbal Zanthoxylum acanthopodium

doi: 10.1016/j.sjbs.2021.02.047

Figure Lengend Snippet: Histology of hypertension rats placental given Extra virgin olive oil (EVOO) (T1) A. Cytochrome c (20 µm), B. FasL (20 µm), C. Apoptosis (20 µm). D. Cytochrome c (20 µm), E. FasL (20 µm), F. Apoptosis (20 µm). EC: Epithelial Cells, FV: Fetal vessels. SA: Spiral artery.

Article Snippet: FasL detection used Fas-L mouse monoclonal antibodies (NOK-1):sc-19681 (dilution 1:50 with PBS, Santa Crus Biotechnology, Santa Cruz, CA, USA), and cytochrome c detection used a monoclonal mouse anti-cytochrome C antibody (ready to use) 7H8.2C12 (Medaysis Enable Innovation Company), formulation in PBS pH 7.4, containing BSA and ≤0.09% sodium aide (NaN 3 ).

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: Histological changes in placental rat apoptosis via FasL and cytochrome c by the nano-herbal Zanthoxylum acanthopodium

doi: 10.1016/j.sjbs.2021.02.047

Figure Lengend Snippet: Histology of hypertension rats placental given nanoherbal ZA (T2) A. Cytochrome c (20 µm), B. FasL (20 µm), C. Apoptosis (20 µm). D. Cytochrome c (20 µm), E. FasL (20 µm), F. Apoptosis (20 µm). EC: Epithelial Cells, FV: Fetal vessels.

Article Snippet: FasL detection used Fas-L mouse monoclonal antibodies (NOK-1):sc-19681 (dilution 1:50 with PBS, Santa Crus Biotechnology, Santa Cruz, CA, USA), and cytochrome c detection used a monoclonal mouse anti-cytochrome C antibody (ready to use) 7H8.2C12 (Medaysis Enable Innovation Company), formulation in PBS pH 7.4, containing BSA and ≤0.09% sodium aide (NaN 3 ).

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: Histological changes in placental rat apoptosis via FasL and cytochrome c by the nano-herbal Zanthoxylum acanthopodium

doi: 10.1016/j.sjbs.2021.02.047

Figure Lengend Snippet: Histology of hypertension rats placental given EVOO and nanoherbal ZA (T3). A. Cytochrome c (20 µm), B. FasL (20 µm), C. Apoptosis (20 µm). D. Cytochrome c (20 µm), E. FasL (20 µm), F. Apoptosis (20 µm). EC: Epithelial Cells, FV: Fetal vessels.

Article Snippet: FasL detection used Fas-L mouse monoclonal antibodies (NOK-1):sc-19681 (dilution 1:50 with PBS, Santa Crus Biotechnology, Santa Cruz, CA, USA), and cytochrome c detection used a monoclonal mouse anti-cytochrome C antibody (ready to use) 7H8.2C12 (Medaysis Enable Innovation Company), formulation in PBS pH 7.4, containing BSA and ≤0.09% sodium aide (NaN 3 ).

Techniques:

Journal: Saudi Journal of Biological Sciences

Article Title: Histological changes in placental rat apoptosis via FasL and cytochrome c by the nano-herbal Zanthoxylum acanthopodium

doi: 10.1016/j.sjbs.2021.02.047

Figure Lengend Snippet: The Positive index of cytochrome c expression on placental histology.

Article Snippet: FasL detection used Fas-L mouse monoclonal antibodies (NOK-1):sc-19681 (dilution 1:50 with PBS, Santa Crus Biotechnology, Santa Cruz, CA, USA), and cytochrome c detection used a monoclonal mouse anti-cytochrome C antibody (ready to use) 7H8.2C12 (Medaysis Enable Innovation Company), formulation in PBS pH 7.4, containing BSA and ≤0.09% sodium aide (NaN 3 ).

Techniques: Expressing